作者：中华医学网发布时间：2017-10-14 07:00浏览：
次
（一） 磷酸盐缓冲液
|
PH |
0.2MNa2HPO4 |
0.2MNaH2PO4 |
PH |
0.2MNa2HPO4 |
0.2MNaH2PO4 |
|
5.8 |
8.0 |
92.0 |
7.0 |
61.0 |
39.0 |
|
5.9 |
10.0 |
90.0 |
7.1 |
67.0 |
33.0 |
|
6.0 |
12.3 |
87.7 |
7.2 |
72.0 |
28.0 |
|
6.1 |
15.0 |
85.5 |
7.3 |
77.0 |
23.0 |
|
6.2 |
18.5 |
81.5 |
7.4 |
81.0 |
19.0 |
|
6.3 |
22.5 |
77.5 |
7.5 |
84.0 |
16.0 |
|
6.4 |
26.5 |
73.5 |
7.6 |
87.0 |
13.0 |
|
6.5 |
31.5 |
68.5 |
7.7 |
89.5 |
10.5 |
|
6.6 |
37.5 |
62.5 |
7.8 |
91.5 |
8.5 |
|
6.7 |
43.5 |
56.5 |
7.9 |
93.0 |
7.0 |
|
6.8 |
49.0 |
51.0 |
8.0 |
94.7 |
5.3 |
|
6.9 |
55.0 |
45.0 |
|
|
|
(二)醋酸-醋酸钠缓冲液(0.2M)
|
PH 18℃ |
0.2MNaAc 毫升 |
0.2MHAc 毫升 |
PH 18℃ |
0.2MNaAc 毫升 |
0.2MNaAc 毫升 |
|
3.6 |
0.75 |
9.25 |
4.8 |
5.90 |
4.10 |
|
3.8 |
1.20 |
8.80 |
5.0 |
7.00 |
3.00 |
|
4.0 |
1.80 |
8.20 |
5.2 |
7.90 |
2.10 |
|
4.2 |
2.65 |
7.35 |
5.4 |
8.60 |
1.40 |
|
4.4 |
3.70 |
6.30 |
5.6 |
9.10 |
0.90 |
|
4.6 |
4.90 |
5.10 |
5.8 |
9.40 |
0.60 |
(三)巴比妥钠-盐酸缓冲液(18℃)
|
PH |
0.04M巴比妥钠溶液ml |
0.2N盐酸 (毫升) |
PH |
0.04M巴比妥钠溶液ml |
0.2N盐酸 (毫升) |
|
6.8 |
100 |
18.4 |
8.4 |
100 |
5.21 |
|
7.0 |
100 |
17.8 |
8.6 |
100 |
3.82 |
|
7.2 |
100 |
16.7 |
8.8 |
100 |
2.52 |
|
7.4 |
100 |
15.3 |
9.0 |
100 |
1.65 |
|
7.6 |
100 |
13.4 |
9.2 |
100 |
1.13 |
|
7.8 |
100 |
11.47 |
9.4 |
100 |
0.70 |
|
8.0 |
100 |
9.39 |
9.6 |
100 |
0.35 |
|
8.2 |
100 |
7.21 |
|
|
|
(四)柠檬酸-柠檬酸钠缓冲液(0.1M)
|
PH |
0.1M柠檬酸ml |
0.1M柠檬酸钠ml |
PH |
0.1M柠檬酸ml |
0.1柠檬酸钠ml |
|
3.0 |
18.6 |
1.4 |
5.0 |
8.2 |
11.8 |
|
3.2 |
17.2 |
2.8 |
5.2 |
7.3 |
12.7 |
|
3.4 |
16.0 |
4.0 |
5.4 |
6.4 |
13.6 |
|
3.6 |
14.9 |
5.1 |
5.6 |
5.5 |
14.5 |
|
3.8 |
14.0 |
6.0 |
5.8 |
4.7 |
15.3 |
|
4.0 |
13.1 |
6.9 |
6.0 |
3.8 |
16.2 |
|
4.2 |
12.3 |
7.7 |
6.2 |
2.8 |
17.2 |
|
4.4 |
11.4 |
8.6 |
6.4 |
2.0 |
18.0 |
|
4.6 |
10.3 |
9.7 |
6.6 |
1.4 |
18.6 |
|
4.8 |
9.2 |
10.8 |